Skip to main content
. 2008 Sep 17;105(38):14353–14358. doi: 10.1073/pnas.0807537105

Fig. 5.

Fig. 5.

PDGF-induced a highly polarized Rac activity in MEF cells, which is mediated by Src. (A) (i Upper) Emission ratio images of the ECFP/YPet-based Rac biosensor in response to 50 ng/ml PDGF in MEF cells seeded on Fibronectin-coated glass lower dishes. (Lower) The DIC images of the cell shown in the top. (ii and iii, Upper) Emission ratio images of the ECFP/YPet-based Rac biosensor before and after PDGF stimulation in (ii) a MEF cell pretreated with 10 μM PP1 for 1 h or (iii) a Src/Yes/Fyn triple-knockout (SYF−/−) MEF cell. (Lower) The DIC images of the cell shown in the top. (B) Emission ratio images of the ECFP/YPet-based Rac biosensor in a MEF cell cultured on fibronectin-coated stripes (10 μm in width) before and after PDGF stimulation for various periods of time. (C) Emission ratio images of the ECFP/YPet-based Rac biosensor in response to PDGF in a MEF cell pretreated with 10 μM PP1 for 1 h, or in a Src/Yes/Fyn triple-knockout (SYF−/−) MEF cell. (D) Bar graphs represent the emission ratios of the ECFP/YPet Rac biosensor averaged over subcellular regions divided along the migration direction on Fn stripes as indicated before and after PDGF stimulation, in control or PP1 pretreated MEF cells, or in SYF−/− cells. (E) Bar graphs representing the ratio of Rac activities at leading edge over the cell rear (mean ± SEM, n = 4) in cells with the same conditions as in D. “*” and “#” indicates the significant difference between groups. (Scale bars, 30 μm.)