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. 2008 Sep 15;105(38):14447–14452. doi: 10.1073/pnas.0803790105

Fig. 4.

Fig. 4.

The absence of Sirt3 selectively affects Complex I activity. (A and B) Representative rate of oxygen consumption using Complex I-dependent substrates for liver mitochondria obtained from a wild-type (A) or Sirt3−/− (B) mouse. (C) Calculated State 3 respiration rate for Complex I from intact liver mitochondria of four wild-type and four Sirt3−/− mice (mean ± SD; *, P < 0.02). (D) Calculated State 3 respiration rate for Complex II-dependent substrate (succinate + rotenone) in wild-type and knockout mice. NS, not significant. (E) Rates of NADH consumption for purified HeLa cell mitochondria after in vitro incubation with deacetylase buffer containing purified Flag-Sirt4 (green), Flag-Sirt3 (red), or Flag-vector (purple). Normalized NADH absorbance was monitored at 340 nm. The arrow indicates the time of addition of rotenone (4 μM final concentration). Shown is the mean rate (±SD) of NADH consumption of triplicate determinations from one of two similar experiments.