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. 2008 Sep 17;105(38):14727–14731. doi: 10.1073/pnas.0805089105

Fig. 1.

Fig. 1.

Arabidopsis PAS2 and yeast PHS1 are functionally exchangeable. (A) Induction of PAS2 expression in haploid cells carrying mutated phs1 allele and pGAL:PAS2 construct (D1B in presence of galactose [Gal]) complemented phs1 phenotype (D1B in presence of glucose [Glc]). Wild-type PHS1 (D1A) cells were grown on Gal- or Glc-supplemented medium. (B) Expression of PAS2 (D1B Gal) prevents free t18:0 and d18:0 accumulation observed in phs1 (D1B glc). (C) Expression of 35S:PHS1 complemented pas2–1 developmental defects. (Scale bar, 1 mm.) (D) Mutation in PAS2 gene led to LCB and phosphorylated LCB (LCB-P) accumulation that was prevented by the expression of PHS1. LCB values are the average of three samples ± SD.