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. 2008 Sep 17;105(38):14545–14550. doi: 10.1073/pnas.0807298105

Fig. 3.

Fig. 3.

NS3 helix α0 mediates membrane association. (A) Sequence analyses of the NS3 amino acid 10–24 segment. See Fig. 1 legend for details. (B) Membrane association of NS3 helix α0-GFP fusion construct. Constructs NS310–24-GFP or NS310–24mut-GFP (see SI Text for details) were expressed in U-2 OS cells and examined by fluorescence microscopy and membrane flotation analyses. Monoclonal antibody JL-8 against GFP was used for immunoblot. Membranes float to the upper, low-density fractions (left portion of the blots). (C) Membrane association of NS3-4A single-chain constructs. Constructs scNS3–4A, scNS310–24mut-4A, scNS3P-4A, and scNS3P10–24mut-4A (see SI Text for details) were examined by immunofluorescence microscopy and membrane flotation by using monoclonal antibody 1B6 against NS3 (3).