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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Curr Opin Biotechnol. 2008 Jul 24;19(4):404–409. doi: 10.1016/j.copbio.2008.06.006

Figure 1.

Figure 1

Current peptide-centric mass spectrometry-based phosphoproteomics methods digest proteins to peptides, resulting in loss of protein state information, although phosphorylation-site specific quantification is possible. In the future, in order to understand how coordinated phosphorylation affects protein function and signaling, it will be necessary to analyze either intact proteins or very large peptides (similar to those that could be generated with a CNBr cleavage). These proteins would then be analyzed by LC-MS/MS with ETD to provide sequence and phosphorylation site occupancy.