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. 1982 Jan;41(1):66–77. doi: 10.1128/jvi.41.1.66-77.1982

Biochemical evidence of recombination within the unsegmented RNA genome of aphthovirus.

A M King, D McCahon, W R Slade, J W Newman
PMCID: PMC256726  PMID: 6283129

Abstract

Four different pairs of temperature-sensitive mutants, derived from the same strain of aphthovirus, were crossed by using an infectious center recombination test. Each parental mutant carried an unselected marker affecting the isoelectric point of a virus-coded polypeptide; progeny of the crosses, able to grow at the nonpermissive temperature, were screened for these unselected markers. Polypeptide charge was shown to be a stable, inheritable character, and three of the four crosses yielded genetic recombinants that possessed the polypeptide marker from both parents. Peptide fingerprinting and high-resolution isoelectric focusing of the polypeptides ruled out the explantation that these viruses were generated by mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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