Figure 3.

Determination of minimal concentration of antibody m74–2 for blocking the formation of tubular networks. VEC-DIC images of cytoplasmic extract supplemented with 125 μg/ml (A), 62.5 μg/ml (B), 31.5 μg/ml (C), 15.5 μg/ml (D), 7.5 μg/ml (E), and 0 μg/ml (F) monoclonal antibody m74–2. Tubular network formation was completely blocked at an antibody concentration of 15.5 μg/ml (D). Short membrane tubules were occasionally visible at an antibody concentration of 7.5 μg/ml (E), and a fully formed network was present in the control samples (F).