TABLE 3.
Ninety-one selected cytochrome P450s (CYP), esterases (CCE), or glutathione transferases (GST) and various oxidase genes and associated primers and conditions for PCR amplification
| Gene | Vector base | Contig | Forward primer | Reverse primer | Ta | Prod. size |
|---|---|---|---|---|---|---|
| Polymorphic by SSCP | ||||||
| Esterases | ||||||
| CCEae1oa | AAEL004341 | 1.115 | AGGTCCAGAAGCCAAACAGG | GGAAGCAGGTGTAGGTTCAGAGTA | 61 | 394 |
| CCEae1A | AAEL005113 | 1.142 | CAAGAGTTGCCACTGGATGA | ATAGGCTGCCATTACGAACA | 52 | 147 |
| CCEae5A | AAEL005123 | 1.142 | ACTTGGCTTTCTTTGCCTACC | CCGCTTTGAGCGATTACTTTAT | 52 | 222 |
| CCEjhe1F | AAEL005200 | 1.145 | TTGTGATGAGTGGGAATGCG | TCGGTACAGTGTCAATGGGTCT | 57 | 218 |
| CCEunk7oa | AAEL008757 | 1.347 | ACGGAGTCTTTCTTGAAGGGTAA | CACTAACTGCACGAAGCGATG | 61 | 318 |
| CCEunk4o | AAEL001517 | 1.35 | CTGCTGCTGCCACATACC | CGCCGTGCTTCCAATC | 57 | 347 |
| CCEae2Da | AAEL010389 | 1.474 | CGAGCGATTGTTATGTCTGG | AGCCCTTCTTCCGAGTTTC | 57 | 344 |
| CCEae4B | AAEL002376 | 1.55 | AAACCCGAAGTGGCTTGT | GCTCTTGGTAACCGTGGC | 61 | 359 |
| CCEae6B | AAEL002378 | 1.55 | CCATCGCAAAGCACCAG | TCAATAGCAAGCATTCCCTC | 61 | 205 |
| CCEbe2oa | AAEL012509 | 1.704 | AGAAATCCGCCGAGAAAGC | GGAGTAAGGGAACCTGATGGAA | 61 | 267 |
| NS-COE1 | AAEL003201 | 1.81 | TATGACTAATCTTCCCATCACTCCA | GATAATGACCCGCAACCAAGT | 57 | 212 |
| Cytochrome P450s | ||||||
| CYP304C1 | AAEL014413 | 1.1104 | CTTCCATTCGGTGCGGGTAA | TGTCGTCGTGGTTAAAGGGTTAGTT | 61 | 277 |
| CYP301A1 | AAEL014594 | 1.1181 | CAAGAGTGCCACAAAGCGTATGA | CAATCACGGTACTGTAAACCCTAAGAA | 61 | 338 |
| CYP9J24 | AAEL014613 | 1.1188 | ACTTCGCTGCTCTACACTT | TACTTGATTCCGTTTCCTT | 52 | 211 |
| CYP9J26 | AAEL014609 | 1.1188 | GAAACGGCGGAGCAT | GTCCACCTTGATTCCAAAA | 61 | 370 |
| CYP9J27v1 | AAEL014616 | 1.1188 | GATTCGCCACCGTTCA | GAGTTTCGGATACCACCAT | 61 | 202 |
| CYP9J28 | AAEL014617 | 1.1188 | TTTGCGAACGATGTGAT | GTTCCCGCTCCCTAAT | 52 | 263 |
| CYP6F2 | AAEL014678 | 1.1219 | CGAACGCAGCAAACAATGGC | CGTGTTGTAGCGGGCAAGGAT | 61 | 305 |
| CYP6BB2a | AAEL014893 | 1.1327 | GTAGTCGCTAAGGACGGAGGAGA | GATCGTGGTGCATCGAGTGG | 61 | 240 |
| CYP6CD1 | AAEL005006 | 1.138 | GAAGTTGACCGTTGGATGCG | TGGTGTAATCCGTAGGCTTTCC | 61 | 252 |
| CYP325R1a | AAEL005775 | 1.174 | GTGCTACGGTGTTCCTTGGT | CCTTTCGGTTGGGTTTCC | 61 | 214 |
| CYP9J10 | AAEL006798 | 1.221 | AGGGTTCGTTGAGGCA | GGTTTACCGTTCAGTGATTT | 57 | 389 |
| CYP9J15 | AAEL006795 | 1.221 | AAGGGCAACCTGAAGCACTCTG | TCCTCGTACAAACGGGACTGAA | 61 | 359 |
| CYP9J19a | AAEL006810 | 1.221 | TGGACGGATAATGAGTTGA | TGTCTATGGTCGGAGGC | 57 | 266 |
| CYP9J8v1 | AAEL006811 | 1.221 | CTTCGCTGCCGTTATCT | TTGACACCGATTTGCTTG | 57 | 381 |
| CYP6AG5 | AAEL006984 | 1.231 | CTAACGAAGTCGGAGGAACG | GGGCAATGGACGAAATGA | 57 | 287 |
| CYP4H32a | AAEL007812 | 1.283 | CGCTGCGATTACTTCCACC | TCGAACGACCGTCATCTTCA | 61 | 282 |
| CYP9M4 | AAEL001320 | 1.29 | CGTCGCAGCCTTCTTAGTTT | CCCAATGATAGGGTCCACCT | 57 | 384 |
| CYP4C52a | AAEL008023 | 1.295 | TTCGACATCGTGCCCTTCAC | TTCTTGCGACAGCAACTGGTTA | 61 | 255 |
| CYP4G35a | AAEL008345 | 1.318 | GCTTGGCGATGGGCTGTT | CATAATCAAATCCCTCCTTGCTCT | 57 | 304 |
| CYP6P12V2a | AAEL014891 | 1.327 | GGCAGGTCACCAACAAGCA | ATCACCGATTCCCGCTCCC | 57 | 380 |
| CYP9J32a | AAEL008638 | 1.352 | TCGGGTAATGGGACTAA | GACTCCGTTGTTTCTTGTT | 52 | 211 |
| CYP6AL1 | AAEL008889 | 1.354 | GCGGCTCAGGTGTTTGTG | CGGCTGTGCCTTTGTCTAT | 61 | 350 |
| CYP6N9 | AAEL009121 | 1.371 | GAAAGCATCCGCCAGCAT | GCAAGGTCGCCAATCCC | 63 | 354 |
| CYP9M8a | AAEL009591 | 1.41 | ATTGAGTGGACCGATTACGACA | GGATTCGGGAAGAAATGTGG | 61 | 358 |
| CYP6AL3a | AAEL009656 | 1.415 | ATTTGATTACTTCGCTCCGTTTGG | TGCCTGGATGTCCTGATGTCG | 61 | 291 |
| CYP6AG8 | AAEL015654 | 1.4359 | TGACTGTTCTCCCGAGTGTTG | GGTTTAGCGAAAGGGTGGC | 61 | 338 |
| CYP4AR2 | AAEL010154 | 1.457 | CACTTCGCATGTTCCCTCC | TCTTAGTGGCATTTCGTCCTG | 61 | 208 |
| CYP325Z1 | AAEL010273 | 1.467 | GGTGGGCAACGAAACATCTAC | AGCAGCAAACGGGACAGC | 57 | 271 |
| CYP12F5a | AAEL001960 | 1.47 | TTTGGAAGTCACTGGCGTAAT | TTGGGCATCGGTGTTTGT | 57 | 233 |
| CYP305A5 | AAEL002043 | 1.48 | GGATTGTACGCTGGGAGGA | AACTTTGTATGGCTTTGGTGAGA | 61 | 256 |
| CYP302A1 | AAEL011463 | 1.581 | AAAAGACTGCCGTTGAGTTGG | GAGATAGGGTTGAGCCGAAGAG | 61 | 245 |
| CYP303A1 | AAEL012144 | 1.66 | TTGGTGAAGGCAGGAAACG | TGAATGGAAAGTGGCTGAAAA | 57 | 282 |
| CYP6BY1a | 1.7 | TCACGCCGCTGTTCCTATT | CCGCCACTTGTTACCTTCG | 61 | 247 | |
| CYP6P12V1a | AAEL012491 | 1.702 | GGACAGTCGGAAGCGGGAGT | GCAGCAGCAAGTTCATAAAGTCGT | 61 | 311 |
| CYP325M1 | AAEL012773 | 1.738 | TTGCCGACGGGCTCAA | TGGCGGGTCCTAAAGATGA | 61 | 210 |
| CYP4H30a | AAEL003399 | 1.85 | CAATCCGCATGTTCAAGACAA | AATCGCTGACCAATACAGTTCC | 61 | 264 |
| CYP4J13 | AAEL013555 | 1.869 | ATGGAGTCTACAATCTGTTTGGGTAT | AACCTGCTGCGGTCGTGTC | 61 | 319 |
| CYP9AE1a | AAEL003748 | 1.96 | CATCCTTATCGGCTTCCAGTTTT | AAGGTGGCGAGGTTTCTGCT′3 | 61 | 392 |
| Glutathione-S-transferases | ||||||
| GSTx1 | AAEL000092 | 1.1 | CCCAAATCAACCGAGTTCTTCA | GCCTGGTCCTCCTCGTATCCT | 61 | 322 |
| GSTd2 | AAEL001078 | 1.22 | TGGCGTTGAGTTGAATCCG | TGCCTCCGCAAATCGTG | 61 | 268 |
| GSTe2 | AAEL007951 | 1.291 | CACCCTGTCGGGCAGTGGAA | TTGGCTTGCTTAACCAGTTCTTTC | 52 | 308 |
| GSTe4 | AAEL007962 | 1.291 | TATTGGTTTGGACCTGGATGTT | ATAGATGATGATGGCGTGGC | 57 | 229 |
| GSTe5 | AAEL007964 | 1.291 | TATGGGAAGGACGATAGTTTGTA | AAGTCATCGGTCAGGGCATC | 57 | 279 |
| GSTe6 | AAEL007946 | 1.291 | CGATGCGGTTCTGCCGATAA | GCCAATTTCTTTGCACCTTCTC | 52 | 208 |
| GSTe7a | AAEL007948 | 1.291 | TGTTTCCGAGACTGCG | TGACTGTTCCATCCGTTTA | 52 | 372 |
| GSTt3 | AAEL009020 | 1.362 | CAGAACGTGAACCGCTTTGGG | ACCTTCGCCTGATTGACTTCCA | 61 | 260 |
| GSTz1 | Unannotated | 1.632 | AGCGTGCCAAGGTTCGGGAGA | GGATGATCGGATACGGGCGTAGAT | 61 | 276 |
| Monomorphic by SSCP | ||||||
| Esterases | ||||||
| CCEunk5o | AAEL000546 | 1.1 | CACGGAGATGATCTGGGCTAC | GCTTCAAGACGGGAACACCTAT | 57 | 308 |
| AaeCOE-15 | AAEL004022 | 1.105 | GATGACGGGCGTGGATTG | GGCTGTTACTATGGCAGGAAGAT | 61 | 372 |
| CCEae4A | AAEL005101 | 1.142 | CGAACTGACTCGGCTACTTTC | TGCTGCCCTTCTTTACGG | 50 | 248 |
| CCEglt3H | AAEL000905 | 1.18 | TGGTGCTGTCGCTGTTTCTG | CGTAAGGGTATCCACGGCTAAT | 61 | 275 |
| CCEae3B | AAEL002385 | 1.55 | GCAACCCTCCAAGCCTTAA | CACCATTGTCCTTCCTACGC | 57 | 359 |
| Cytochrome P450s | ||||||
| CYP325AA1 | AAEL004012 | 1.105 | AACGATGCGGCTCTACCCA | GAACTTCAAATCCTTTCGAGTCAAT | 57 | 346 |
| CYP6CB2 | AAEL002872 | 1.1337 | ATTGCCCATTCGTGCTCTG | TCTCCTCCGTGTTCCCTTTT | 57 | 282 |
| CYP6AK1 | AAEL004941 | 1.136 | TCTCGAAAGACAATGCAGACAAC | TCCAAATCGGCGTACAATCC | 61 | 229 |
| CYP325X1 | AAEL005695 | 1.17 | TGATACCGAGGAGGAATACAATAAAC | GGGCCACATAAGGCACAACC | 61 | 335 |
| CYP325Q1 | AAEL006044 | 1.187 | GAATGATGAAACCGATGACG | GCACTCCTCCGCAAAGC | 61 | 386 |
| CYP307B1v2 | AAEL006875 | 1.224 | CTCAACAACTACGACCTCAGCAT | GAGCCCACCGGAACGAA | 61 | 248 |
| CYP4H29 | AAEL007830 | 1.285 | TTCATCCATCCGTTCCAATCA | TCTATCGTGGCACAATCTTCACA | 61 | 393 |
| CYP49A1 | AAEL008638 | 1.338 | CAGAACCCAGACAAGCAGGAA | CCGTAACCGAAGGGCAAACTA | 61 | 2000 |
| CYP307A1 | AAEL009762 | 1.427 | GGCGAAGACGAACCAGAGCG | GGCGATGAGGAGTACCGAAGGA | 61 | 308 |
| CYP9M9 | AAEL001807 | 1.43 | CGGCACCAAGGTAACGG | CATCGGCACATCGGTCTTT | 61 | 280 |
| CYP12F6 | AAEL002005 | 1.47 | ATGATTCTGGCAGGTGTC | GGTTTCCTACTATGGGTGG | 52 | 220 |
| CYP314A1 | AAEL010946 | 1.524 | TCCGATACAATCACCAACAATG | AATGGAAGCACCAAAGACGA | 57 | 305 |
| CYP325S1 | AAEL000326 | 1.6 | TGAATTTCTACGGAATATGGAAGG | AACGGACGACTGCTGTTGG | 57 | 279 |
| CYP315A1 | AAEL011850 | 1.622 | TTTGAAAGGATAGTGCCAGAA | GGAGCAATAGGGAACAGACG | 57 | 323 |
| CYP6AA5 | AAEL012492 | 1.702 | GCCCGTCATTCGCACAGT | TTCAACATCCAGCCAAAGTCC | 61 | 210 |
| CYP4H33 | AAEL013798 | 1.923 | CCTGATTCGCACTACCTGTG | AACTCATCCTTTCCTGCCTTA | 61 | 395 |
| CYP4J17 | AAEL014019 | 1.985 | CCAGGAGGAATGCGACAAACT | ACCTAACAGGCGGTAGGGAAAT | 57 | 250 |
| Glutathione-S-transferases | ||||||
| GSTt4 | AAEL004229 | 1.111 | CCAACGGCGTCACCATTCTC | GCTGATTCAGGTTCTGCTCCATCT | 61 | 246 |
| GSTe1 | AAEL007954 | 1.291 | TTTCCTTTCCCTAAGTTCCGTCTC | CAGCACAAACCCATCATCGTC | 49 | 179 |
| GSTe3 | AAEL007947 | 1.291 | CTGGTACAGAAATACGGCAAAGA | GAACAGGGCATTCAGAGTGGA | 57 | 297 |
| GSTx2 | AAEL010500 | 1.481 | CGAGGCATCATCAACCAACGG | TCGGCAGATCACTCAGGTCAACA | 63 | 268 |
| GSTi1 | AAEL011752 | 1.608 | GCCGCTTGGGAAGTAAGTTTG | TGATGGGATGAATTGGGTGCT | 61 | 255 |
Ta, annealing temperature; Prod. size, product size (bp).
Genes that were placed on the linkage map in this study.