Figure 1.—

Overexpression of MoDcl1 cDNA partially restored hairpin RNA-induced gene silencing in the MoDcl2 KO mutant. The overexpression (OE) vectors of cMoDcl1 and cMoDcl2 were introduced into the GFP-expressing MoDcl2 knockout mutant by the cotransformation method with the GFP silencing construct, pEGFP-SA-neo carrying the geneticine resistant gene (Kadotani et al. 2004). As a control, pEGFP-SA-neo was introduced into a GFP-expressing strain in a wild-type background. The resulting transformants were classified into five categories (0–20%, 21–40%, 41–60%, 61–80%, and >80%) on the basis of relative fluorescence to the parent strain, and the number of the transformants in each category was plotted in the graph. The full-length cDNAs of the MoDcl1 and MoDcl2 genes were obtained by reverse transcription with oligo dT primers and SuperScript III (Invitrogen, San Diego) and subsequent 5′-RACE.