Figure 2.—

Northern blot analysis of GFP siRNAs in the MoDcl1 and MoDcl2 overexpressing transformants. Three MoDcl1 and MoDcl2 overexpressing transformants each were subjected to siRNA analysis. Low-molecular-weight RNA was extracted and hybridized with a GFP probe as described previously (Kadotani et al. 2004). DNA oligonucleotides (30 and 21 mer) were used as molecular size markers. Equal loading of total RNA was estimated by ethidium bromide staining of predominant RNAs. M, oligonucleotide markers; C, parent strain (GFP-expressing MoDcl2 knockout mutant); tMoDcl1-OE11–13, transformants with the cMoDcl1 OE vector; tMoDcl2-OE21–23, transformants with the cMoDcl2 OE vector.