TABLE 1.
Yeast strains
| Strain | Genotype | Figures |
|---|---|---|
| YSB348a | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3b | 1B, 6B, 6C |
| CGY101 | YSB348 pRS425 | 1C, 2A, 2C, 3A, 4C, 6A |
| CGY102 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(300L)∷mURA3-HIS3 pRS425 | 3A, 3B |
| CGY103 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(600L)∷mURA3-HIS3 pRS425 | 3A, 3B |
| CGY111 | YSB348 pJOE31 | 1C, 2B, 2C, 3A, 3B, 4C, 6A |
| CGY112 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(300L)∷mURA3-HIS3 pJOE31 | 3A, 3B |
| CGY113 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(600L)∷mURA3-HIS3 pJOE31 | 3A, 3B |
| CGY129 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 sir2Δ∷kanMX4 pRS425 | 4C |
| CGY130 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 sir2Δ∷kanMX4 pJOE31 | 4C |
| CGY132 | MATα his3Δ200 leu2Δ1 ura3-167 trp1Δ∷mURA3-HIS3 pRS425 | 3A, 3B |
| CGY133 | MATα his3Δ200 leu2Δ1 ura3-167 trp1Δ∷mURA3-HIS3 pJOE31 | 3A, 3B |
| CGY145 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 npt1Δ∷kanMX4 | 6B, 6C |
| CGY146 | CGY145 pRS425 | 6A |
| CGY147 | CGY145 pJOE31 | 6A |
| CGY164 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 sir4Δ∷kanMX4pRS425 | 4C |
| CGY153 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 bna1Δ∷kanMX4 | 6B |
| CGY165 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 sir4Δ∷kanMX4 pJOE31 | 4C |
| CGY166 | CGY153 pRS425 | 6A |
| CGY167 | CGY153 pJOE31 | 6A |
| DSY35c | MATahis3Δ200 leu2Δ0 lys2Δ202 trp1Δ63 ura3-52 ADH4∷URA3-TEL pRS425 | 4A |
| DSY37c | MATahis3Δ200 leu2Δ0 lys2Δ202 trp1Δ63 ura3-52 ADH4∷URA3-TEL pJOE31 | 4A |
| JS1153c | MATahis3Δ200 leu2Δ0 lys2Δ202 trp1Δ63 ura3-52 ADH4∷URA3-TEL sir2Δ∷kanMX4 pRS425 | 4A |
| JS1154c | MATahis3Δ200 leu2Δ0 lys2Δ202 trp1Δ63 ura3-52 ADH4∷URA3-TEL sir2Δ∷kanMX4 pJOE31 | 4A |
| JM98 | MATα his3Δ200 leu2Δ1 met15Δ0 ura3-167 RDN1(50L)∷mURA3-HIS3hst1Δ∷kanMX4 | 6D |
| JM212 | MATα his3Δ200 leu2Δ1 met15Δ0 ura3-167 RDN1(50L)∷mURA3-HIS3 nrk1Δ∷kanMX4 tna1Δ∷kanMX4 | 6B |
| JM234 | MATα his3Δ200 leu2Δ1 met15Δ0 ura3-167 RDN1(50L)∷mURA3-HIS3 nrk1Δ∷kanMX4 bna1Δ∷kanMX4 | 6B |
| JM236 | MATα his3Δ200 leu2Δ1 met15Δ0 ura3-167 RDN1(50L)∷mURA3-HIS3 nrk1Δ∷kanMX4 npt1Δ∷kanMX4 | 6B |
| JM238 | JS932 pRS425 | 6A |
| JM240 | JS932 pJOE31 | 6A |
| JM242 | JS944 pRS425 | 6A |
| JM244 | JS944 pJOE31 | 6A |
| JM254 | JM236 pRS425 | 6A |
| JM256 | JM236 pJOE31 | 6A |
| JM265d | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrΔA∷TRP1 pRS425 | 4B |
| JM267d | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrΔA∷TRP1 pJOE31 | 4B |
| JM269d | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrΔA∷TRP1 sir2∷HIS3pRS425 | 4B |
| JM271d | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrΔA∷TRP1 sir2∷HIS3pJOE31 | 4B |
| JM311 | YSB348 pFR1 | 2C |
| JM313 | YSB348 pJSS95-3 | 2C |
| JM315 | YSB348 pAAH5 | 2C |
| JS902 | MATα his3Δ200 leu2Δ1 ura3-167 pnc1Δ∷kanMX4 | 6B |
| JS932 | MATahis3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 tna1Δ∷kanMX4 | 6B |
| JS944 | YSB348 nrk1Δ∷kanMX4 | 6B |
| JS1011 | MATα his3Δ200 leu2Δ1 met15Δ0 trp1Δ63 ura3-167 RDN1(50L)∷mURA3-HIS3 | 6D |
| JS1041 | MATα his3Δ200 leu2Δ1 met15Δ0 trp1Δ63 ura3-167 RDN1(50L)∷mURA3-HIS3sir2Δ∷kanMX4 | 6D |
| JS1046 | JS1041 pGLC463, pRS424 | 5 |
| JS1047 | JS1041 pGLC463, pJOE30 | 5 |
| JS1048 | JS1041 pGLC26, pRS424 | 5 |
| JS1049 | JS1041 pGLC26, pJOE30 | 5 |
| JS1050 | JS1041 pGLC65, pRS424 | 5 |
| JS1051 | JS1041 pGLC65, pJOE30 | 5 |
| JS1056 | JS1041 pGLC252, pRS424 | 5 |
| JS1057 | JS1041 pGLC252, pJOE30 | 5 |
| JS1098 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 sir3Δ∷kanMX4 pRS425 | 4C |
| JS1099 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(50L)∷mURA3-HIS3 sir3Δ∷kanMX4 pJOE31 | 4C |
| JS1100 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(PRO+-61L)∷mURA3-HIS3 pRS425 | 2D |
| JS1101 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(PRO+-61L)∷mURA3-HIS3 pJOE31 | 2D |
| JS1102 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(proΔ-61L)∷mURA3-HIS3 pRS425 | 2D |
| JS1103 | MATα his3Δ200 leu2Δ1 ura3-167 RDN1(proΔ-61L)∷mURA3-HIS3 pJOE31 | 2D |
| JS1129 | YSB348 pSB766 | 2B, 3B |
| JS1133 | YSB348 pRS425, pRS424 | 3C |
| JS1134 | YSB348 pSB766, pRS424 | 3C |
| JS1135 | YSB348 pRS425, pJOE30 | 3C |
| JS1136 | YSB348 pSB766, pJOE30 | 3C |
a
Strain described in Buck et al. (2002).
b
Other parental strains with mURA3-HIS3 located in unique sequence flanking the rDNA locus were described in Buck et al. (2002).
c
Parental strain JJSy143 was described in Gallo et al. (2004).
d
Parental strain YLS59 was described in Sussel and Shore (1991).