Figure 4.—
Examples of the transgene copy number testing and mature fiber length of the T2 cotton transgenic progeny. (A) Genomic DNA (20 μg/lane) of the wild type and the T2 of the two independent transgenic (AS24-1 and AS54-1) plants was digested with EcoRI (left) and HindIII (right), respectively, transferred to nylon membrane, and hybridized with 32P-labeled NPTII. (Left) WT, wild-type plant; lanes 1–5, five T2 progeny of AS24-1; lane 6, one T2 progeny of AS54-1. Molecular weight markers are indicated in kilobase pairs. (B) Mature fiber lengths of the T2 cotton transformants and wild-type cotton seeds. Measurement of the fiber lengths showed that fiber elongation in the transgenic plants was shorter than that in the wild-type plant. AS24-1-2, one T2 plant of AS24-1 without the transgene copy, displayed a fiber phenotype similar to wild type.