Figure 5.—

Expression profiling of seven genes important for fiber development in wild-type and transgenic plants. Total RNA samples prepared from 0-DPA, 1-DPA, and 3-DPA ovules with their fibers attached from the two GhMYB109 antisense transgenic and wild-type plants were used for qRT–PCR analysis. 18S rRNA was used as an internal control. The expression of the GhACT5 gene appeared not to be affected in the transgenic lines, whereas GhACO1, GhACO2, GhTUB1, and GhACT1 were expressed at lower levels in the transgenic plant than in wild type.