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Suspension growth of wild-type and mutant cells. Cells were inoculated into flasks containing the HL5 medium at a density of around 2 × 105 cells/ml at time 0 and vigorously shaken on a rotary shaker at 21°C. The cell densities were monitored every 12 h. •, Wild-type cells; ○, mhcA− cells; ▪, mhcA− cells expressing recombinant wild-type myosin; ▾, mhc− cells expressing E459V myosin; ♦, mhc− cells expressing E476K myosin; ▴, mhcA− cells expressing F481C/N483K myosin.
