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. 2008 Aug 28;27(19):2567–2579. doi: 10.1038/emboj.2008.173

Figure 5.

Figure 5

Active AurA is required for RanGTP-dependent aster formation. (A) Localization of AurA on MT asters assembled upon RanGTP addition to M-phase egg extract. Endogenous AurA was detected by immunofluorescence with the anti-AurA antibody (left panels). GFP, GFP-wt or GFP-KD added to egg extracts is visualized through the GFP fluorescence. In the merge, AurA and GFP are in green and MTs are in red. Bar, 10 μm. (B) Graphs showing the number of RanGTP-dependent asters formed in mock-depleted (control) extract, AurA-depleted extract (ΔAurA), AurA-depleted extract containing recombinant wt AurA (ΔAurA+wt) or kinase-dead AurA (ΔAurA+KD) and in control extract containing GST-NT. The number of asters in the control was set at 100%. The graphs show the mean of three independent experiments. Error bars stand for s.d. *P<0.05 compared with control. See also Supplementary Figure 4.