Figure 7.

Role of AurA in spindle assembly in cycled egg extract. (A) Representative mitotic structures formed in cycled egg extracts containing sperm nuclei. Percentages of the different types of structures formed in mock-depleted extract (control), AurA-depleted extract (ΔAurA), AurA-depleted extract containing recombinant wt Aurora (ΔAurA+wt) or kinase-dead protein (ΔAurA+KD) (left graph) and in control or AurA-depleted extract (ΔAurA) containing GST-NT (right graph). The graphs show one representative experiment of three. Bars, 10 μm. (B) Representative images of bipolar spindles formed in the different experimental conditions as in (A). Graphs show the quantification of the total MT fluorescence intensity (left) and the length and width (right) of the bipolar spindles averaged from three independent experiments. Control values were set at 100%. Error bars stand for s.d. ^*P<0.05 compared with control. DNA is in blue, MTs are in red. Bars, 10 μm.