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. 2008 Sep 4;27(19):2580–2591. doi: 10.1038/emboj.2008.174

Figure 3.

Figure 3

Transport through the TGN/endosomal system is essential for the secretion and processing of Nodal precursor, but not for cell surface expression. (A) Immunostaining of Flag-tagged Nr at the surface of unpermeabilized COS1 cells treated with or without BFA (5 μg/ml). BFA did not prevent accumulation of uncleaved Nodal precursor at the cell surface, except when de novo synthesis was blocked by cycloheximide (CHX, 5 μg/ml). (B) BFA inhibited the secretion of Nodal into the medium, leading to sequestration of the 47-kDa form in cell lysates.