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. 2008 Sep 11;27(19):2471–2483. doi: 10.1038/emboj.2008.182

Figure 4.

Figure 4

Imaging of movements of LIS1, TUBB3, DIC1 and KLC1 in DRG. Direct visualization of the anterograde movement of fluorescence-tagged proteins in DRG neurons using confocal microscopy. (A) EGFP–LIS1 and mCherry–DIC1. (B) EGFP–TUBB3 and mCherry–DIC1. (C) EGFP–KLC1 and mCherry–DIC. (D) EGFP–KLC1 and mCherry–LIS1. Dotted lines indicate dynamic colocalization. Yellow signal indicates colocalized transport. Calculated speed of particles was distributed in 0.5–1.6 μm/s, consistent with the speed of kinesin.