Table 4.
Demonstration of LIS1-dependent anterograde transport of cytoplasmic dynein in MEF cells
| (A) | |||
|---|---|---|---|
| mDRG cells | mChe–TUBB3/EGFP–DIC1 complex (%) | Free mChe–TUBB3+free EGFP–DIC1 (%) | N |
| Anti-LIS1 (−) | |||
| Anterograde migration | 21 (60) | 14 (40) | 35 |
| Retrograde migration | 17 (49) | 18 (51) | 35 |
| Total | 38 (54) | 32 (46) | 70 |
| Anti-LIS1 (+) | |||
| Anterograde migration | 7 (19) | 29 (81) | 36 |
| Retrograde migration | 19 (51) | 18 (49) | 37 |
| Total | 26 (36) | 47 (64) | 73 |
| (B) | |||
| MEF cells | mChe–TUBB5/EGFP–DIC1 complex (%) | Free mChe–TUBB5+free EGFP–DIC1 (%) | N |
| Anti-LIS1 (−) | 21 (66) | 11 (34) | 32 |
| Anti-LIS1 (+) | 8 (26) | 23 (74) | 31 |
| To address whether LIS1 mediates anterograde transport of cytoplasmic dynein in other type of cells, we examined the effect of an anti-LIS1 antibody on co-migration frequency of mCherry–TUBB3/EGFP–DIC in DRG neurons (A) and MEF cells (B). Note: The presence of an anti-LIS1 antibody selectively diminished co-migration frequency of mCherry–TUBB3/EGFP–DIC in DRG neurons. Even though determination of direction was not possible in MEF cells, an anti-LIS1 antibody reduced co-migration frequency of mCherry–TUBB3/EGFP–DIC also. | |||