Fig. 3.

Intracellular uptake and photodynamic and hyperthermic effects in vitro. The cellular uptake of ZnPc-SWNHox-BSA was investigated by confocal microscopy (excitation λ = 488 nm, emission detected at λ = 510 nm) after incubation of 5RP7 cells (transformed rat fibroblasts) with ZnPc-SWNHox-BSA-AF in the culture medium for 24 h. (A) Fluorescence image. (B) Differential interference contrast (DIC) image. (C) Combined fluorescence and DIC. (D) Flow cytometry of the 5RP7 cells. dashed black line, control; solid black line, incubated with BSA-AF; blue line, incubated with SWNHox-BSA-AF; red line, incubated with ZnPc-SWNHox-BSA-AF. (E) Photodynamic and hyperthermia destruction of 5RP7 cells. The viability of 5RP7 cells was estimated by using the WST-1 assay. The viabilities of the cells with and without laser irradiation (670 nm) for 5 min were indicated as red and blank bars, respectively.