Table 1.

Expression levels of NTR1, D03, and D03-L167R in multiple hosts

	NTR1	D03 (fold of NTR1)*	D03-L167R (fold of NTR1)*
E. coli,† no. per cell	705 ± 101	6,155 ± 777‡ (8.7 ± 1.7)	4,647 ± 879 (6.6 ± 1.6)
P. pastoris,§ pmol/mg	18.3 ± 5.4	216.7 ± 36.9 (11.9 ± 4.1)	51.1 ± 4.3 (2.8 ± 0.9)
HEK293T, no. per cell	18,300 ± 1,300	58,800 ± 6,600 (3.2 ± 0.4)	33,300 ± 5,400 (1.8 ± 0.3)

*Parenthetical terms give the ratio of mutant expression to that of NTR1.

^†Expression in 1 l cultures.

^‡Expression in mutated vector pRG_C7054G.

^§Functional receptor (pmol) normalized to total membrane protein content (mg). NT binding cannot be measured with whole P. pastoris cells.