Fig. 2.

Binding of Hho1p to chromatin increases in stationary phase. (A) Chromatin was prepared from a yeast strain expressing a unique, c-myc tagged Hho1p following growth for the times indicated (lanes 1–8), and ChIP performed using an anti-c-myc antibody. The recovered DNA and the corresponding control input DNA were amplified by PCR using oligonucleotide primer sets to the genes indicated and the PCR products resolved by agarose gel electrophoresis. A representative result is shown (n = 3). (B) ChIP samples were quantitatively amplified by using oligonucleotide primer sets to the SNZ1 and 25S rDNA genes. The determined levels were normalized to that of the corresponding input DNAs and the ratios expressed relative to that of the 4 h time points (average± SD, n = 3). (C) As a control for equal cross-linking efficiency throughout the growth period shown in A, ChIP was performed at the indicated times (lanes 1–8) by using an anti-H4 antibody. The recovered DNA samples were amplified and resolved as in A. A representative result is shown (n = 3).