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EMT and epigenetic modifications at the E-cadherin locus occur de novo. (A) Photomicrographs (10×) of two early- (C1-E and C2-E) and late- (C1-L and C2-L) passage clones. (B) Immunoblot analysis of fibronectin (Fn), N-cadherin (N-cad), and E-cadherin (E-cad) on cell lysates prepared from each clone. (C) Transwell motility assay depicting migration of clones toward MEGM + 10% FBS as a chemoattractant for 48 h. (D) MSP analysis of E-cadherin and 14–3-3σ.
