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. 2008 Sep 22;105(39):14928–14933. doi: 10.1073/pnas.0802355105

Fig. 5.

Fig. 5.

Functional divergence in amniote HoxA-11 genes ability to up-regulate expression from the decidual PRL enhancer. The ability of HoxA-11 genes to activate expression from the decidual PRL enhancer was determined in a luciferase reporter construct using dPRL-332/luc3 (d332/luc3). Transfection with human HoxA-11 alone (HsaHoxA11) repressed reporter gene expression. Transfection with human FOXO1A alone (HsaFOXO1A) had no effect on reporter gene expression. Cotransfection of human FOXO1A with human HoxA-11 (human A11) or mouse HoxA-11 (mouse A11) activated reporter gene expression. Cotransfection of human FOXO1A with opossum HoxA-11 (opossum A11), platypus HoxA-11 (platypus A11) or chicken HoxA-11 (chicken A11) repressed reporter gene expression. Cotransfection of the reconstructed ancestral eutherian HoxA-11 gene (AncEutherian A11) with the human FOXO1A gene activated reporter gene expression, conversely cotransfection of the reconstructed ancestral therian HoxA-11 gene (AncTherian A11) with the human FOXO1A did not lead to activation of reporter gene expression. Expression levels are shown as fold changes relative to luciferase expression in cells transfected with the reporter gene (d332/luc3) and empty vector (pcDNA3.1). n = 6, mean ± SEM.