Fig. 2.

VSV plus HDI combination treatment synergistically increases apoptosis in PC3 cells. PC3 cells were either not treated (NT) or pretreated with MS-275 or SAHA for 24 h then infected with VSV at 0.1 MOI. (A) Induction of apoptosis was measured by Annexin V staining in the presence or absence of the pan-caspase inhibitor Z-VADfmk. (B) Mitochondrial membrane-depolarization was assessed by JC-1 staining. (C) Caspase 8, 3, and 9 cleavage was determined 48 h after VSV infection in the presence or absence of HDIs by immunoblot of whole cell lysates. Cleaved caspase forms are indicated by open arrows.