Fig. 3.

HDIs augments VSV replication through inhibition of the IFN antiviral response. PC3 cells were either not treated (NT) or pretreated with MS-275 or SAHA for 24 h then infected with VSV at 0.1 MOI. (A) IFN-α levels in supernatants were assayed by ELISA at 24 h after infection. (B) Induction of antiviral genes IFN-beta, IRF-7 and Mxa was assessed at different times after infection by RT-PCR. NT denotes non-HDI treated cells (C) IFN-stimulated antiviral genes were analyzed by immunoblot at different times after VSV alone or VSV plus HDI treatment. (D) Different cell lines were pretreated with HDIs for 7 h and then infected with VSV-Δ51-GFP at 0.1 MOI in the presence or absence of recombinant IFNα. GFP expression was monitored 24 h after VSV inoculation.