Fig. 3.

Detection of the spin mutation. (A) The mutation was predicted to introduce a HincII restriction enzyme site in genomic DNA from spin mutant mice. Amplification of Ptpn6 genomic DNA followed by HincII restriction enzyme digest confirmed the presence of the mutation. (B) SHP1 protein levels were normal in purified bone marrow-derived macrophages from spin homozygotes. The Western blot shown in Fig. 2D was reprobed with SHP1 antibodies, or tubulin antibodies as a loading control. Timepoints indicate minutes after stimulation with LPS in vitro.