Table 1.

Site-directed mutagenesis of tumstatin peptides and their action on endothelial cell proliferation

Tumstatin peptide	Amino acid sequence	Endothelial cell inhibition*
T7	TMPFLFCNVNDVCNFASRNDYSYWL	++++
T7-mutant 1 (MIN)	TMPFMFCNINNVCNFASRNDYSYWL	-
T7-mutant 2 (MI)	TMPFMFCNINDVCNFASRNDYSYWL	-
T7-mutant 3 (IN)	TMPFLFCNINNVCNFASRNDYSYWL	+
T7-mutant 4 (N)	TMPFLFCNVNNVCNFASRNDYSYWL	+
T7-mutant 5	TMPFLFCNVNDVCNFASRNDAK	-
T7-mutant 6	TMPFLYCNPGDVCYYASRNDKSYWL	+
T7-mutant 7	TLPFAYCNIHQVCHYAQRNDRSYWL	++

*Percentage inhibition of endothelial cell proliferation (C-PAE cells), relative to tumstatin peptide, at 33 μM concentration. −, no activity; +, <25% of tumstatin peptide activity; ++, 25–50% of tumstatin peptide activity; +++, 50–75% of tumstatin peptide activity; ++++, maximal activity of tumstatin peptide (T7).