Table 1.
The proteins of the loose (L) and firm (F) mucus were separated by PAGE (Fig. S1) and the proteins identified as tryptic peptides by LC-MS/MS
| Protein name | Gene | Loc | L* | F* |
|---|---|---|---|---|
| Anterior gradient 2 | Agr2 | sec | x | x |
| Ca-activated Cl channel 3 | Clca3 | sec | x | x |
| Ca-activated Cl channel 6 | Clca6 | sec | x | x |
| IgG Fc-binding protein | Fcgbp | sec | x | x |
| γ-Glutamyl hydrolase | Ggh | sec | x | x |
| Immunoglobulin | Ig | sec | x | x |
| Lumican | Lum | sec | x | x |
| Mucin 2 | Muc2 | sec | x | x |
| Major urinary proteins 11 and 8 | Mup | sec | x | x |
| Oncoprotein-induced transcript 1 | Oit1 | sec | x | x |
| Mucosal pentraxin | Ptx | sec | x | x |
| Arginyl aminopeptidase | Rnpep | sec | x | x |
| Seminal vesicle secretory protein IV | Svs4 | sec | x | x |
| Zymogen granule membrane protein 16 | Zg16 | sec | x | x |
| Mucin 3(17), orthologue hMUC17 | Muc3(17) | sec-tm1 | x | (x) |
| Vomeroglandin | Dmbt1 | sec-tm1 | x | (x) |
| Polymeric immunoglobulin receptor | Pigr | sec-tm1 | x | (x) |
| Quiescinsulfhydryl oxidase 1 | Qsox1 | sec-tm1 | x | (x) |
| Mucin13 | Muc13 | tm1 | x | x |
| Basigin | Bsg | tm1 | x | x |
| Uvomorulin | Cdh1 | tm1 | x | x |
| Cadherin 17 | Cdh17 | tm1 | x | x |
| CEACAM 1 | Ceacam1 | tm1 | x | x |
| Hephaestin | Heph | tm1 | x | x |
| Mucin-like protocadherin | Mupcdh | tm1 | x | x |
| Nicastrin | Ncstn | tm1 | x | x |
| Protocadherin LKC | Pcdh | tm1 | x | x |
| Tumor-associated Ca signal transducer 1 | Tacstd1 | tm1 | x | x |
| Maltase-glucoamylase | Mgam | tm2 | x | (x) |
Only secreted proteins or transmembrane proteins with a large extracellular domain are listed. A detailed list is presented in Table S1. Loc, localization; sec, secreted protein; tm1, type 1 transmembrane protein; tm2, type 2 transmembrane protein.
*Identification: One peptide at 99% significance level (individual peptide score cutoff = 47) and one supporting peptide at 95% significance level (individual peptide score cutoff = 40) are marked with an x. Proteins only identified by peptides with ion score cutoff = 25 are marked by (x). Multiple entries for the same protein were combined, and identification in two of three analyses of at least one sample was required for inclusion.