Figure 9.

PIPKs and PIP₂ were not associated with invaginations of the nuclear envelope. Invaginations of the nuclear envelope can transverse nuclei (Fricker et al., 1997) and produce a series of dots (arrows) seen here in thin optical sections of methanol-fixed human 2RA fibroblasts labeled with biotin-conjugated Con A (a lectin that binds mannose residues of nuclear envelope glycoproteins). Thin optical sections of the triple-labeling with anti-PIPKIα (A) or anti-PIPKIIα (E) polyclonal antibodies, Con A (C and G) and anti-PIP₂ mAb AM212 (B and F) are shown. The overlay of PIPK and Con A staining patterns is shown in yellow (D and H). Bar, 10 μm.