Fig. 4.

Rotenone inhibition of complex I activity. Mesencephalic neurons were cultured from E14 C57/BL6 mouse embryos for 6 d. (A) Comparison of dose response of rotenone inhibition of complex I and induction of TH⁺ neuron death. Cells were treated on DIV6 with rotenone for 24 h, and complex I activity in intact cells was measured by a NADH dehydrogenase activity assay (n = 7). (B) Mitochondria were freshly purified from cells on DIV6, and complex I activity in mitochondria was measured by the polarography method. Values represent means (n = 3). Error bars represent SEM.