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. 2008 Sep 25;105(39):15178–15183. doi: 10.1073/pnas.0803054105

Fig. 2.

Fig. 2.

Analysis of tetrapyrroles by LC/MS. WT Arabidopsis seedlings were grown for 3 d in complete darkness with or without 5 μM NF, followed by 24 h in WL. Pigments were extracted from aerial tissue and analyzed by LC/MS as described in Experimental Procedures. (A) PDA trace across the complete spectrum (200–800 nm) for the two samples (offset for clarity). Note that the scale for the 5 μM NF sample has been greatly expanded compared to the control (no NF). The regions corresponding to the elution range for the majority of porphyrins and carotenoids are indicated. (B) Total ion current (TIC) chromatogram of the same traces sampled from m/z 200-1000. The inset shows an enlargement of the region corresponding to the elution times for porphyrins. (C) Fluorescence spectra for the same traces at the excitation and emission wavelengths specific for Mg-proto (410 nm and 595 nm respectively). Also shown is the emission spectrum of 1 pmol Mg-proto standard.