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. 2008 Sep 25;105(39):15190–15195. doi: 10.1073/pnas.0806324105

Fig. 3.

Fig. 3.

Hypocotyl elongation and root inhibition were differentially affected by distinct auxin analogs. (A and B) Root inhibition assay. Total root length of 6-day-old seedlings grown in the presence of 533 and 2,4-D (A) and 602 (B). (C) qPCR analysis of 6-day-old seedlings treated with different auxin analogues for 1 h. Fold change compared with mock treatment is indicated on top of the columns. (D and E) Histochemical assay of the DR5:GUS reporter line. (D) Five-day-old DR5:GUS seedlings treated for 16 h with DMSO (Left) or 1 μM 602 (Right). (E) DR5:GUS seedlings grown for 5 days in the presence of the indicated small molecules. Note that whereas 1 μM 602 induced DR:GUS in the shoot, it had no apparent effect in the root. Lateral root initiation is marked by an arrow. (F) qPCR analysis of 2-day-old seedlings treated with different auxin analogues for 1 h. Fold change compared with mock treatment is indicated on top of the columns. Error bars indicate SE.