Figure 3.

Baf A1 inhibits the nocodazole-induced redistribution of Golgi enzymes. Control and Baf A1-treated NRK cells were either fixed directly or after a further 30 or 90 min incubation in the presence of the microtubule-depolymerizing drug nocodazole (5 μM). The cells were stained for immunofluorescence microscopy using antibodies against Golgi mannosidase II, and the number of scattered, peripheral Golgi structures was determined as described in MATERIALS AND METHODS. Figure 1, A and C, also illustrates the typical distribution of mannosidase II in control and Baf A1-pretreated cells, respectively, before the addition of nocodazole (0 min time point).