Figure 6.

Pre-Golgi tubules can also be detected in control and 15°C-treated cells. Control NRK (A) and PC12 cells (A, inset) and NRK cells incubated for 2 h at 15°C (B and inset) were stained with antibodies against rab1p. Note the tubular extensions and connections of the pre-Golgi structures (arrowheads) that become more pronounced in response to the low-temperature treatment. The asterisk in B denotes the Golgi region. (C) The pre-Golgi tubules accumulating in NRK cells at 15°C also contain p58. Bars, 5 μm (A, B, and insets) and 2.5 μm (C).