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Metabolic labeling of chimeric GFPs. COS-7 cells transiently expressing N-terminal fatty acylated GFP chimeras and mutants were metabolically labeled with 125I-IC16 to assess chimera palmitoylation (A) and with [3H-9,10(n)]myristic acid to assess chimera myristoylation (B). (C) Western blot analysis of corresponding transiently transfected COS-7 cells to assess protein production. Plus (+) and minus (−) signs refer to anti-GFP serum and preimmune serum, respectively.
