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Colocalization of various chimeric GFPs with the Golgi marker BODIPY TR-ceramide in living COS-7 cells. GFP intrinsic fluorescence of YesGFP, Yes(C3S)GFP, and Yes(G2A)GFP is shown in A, D, and G, respectively. The distribution of BODIPY TR-ceramide is shown in B, E, and H. The merged images are shown in C, F, and I. Bar, 10 μm.
