Figure 6.

Colocalization of chimeric GFPs with the Golgi apparatus in fixed COS-7 cells. The GFP chimeras were detected by incubation with mouse anti-GFP Ab followed by FITC-conjugated anti-mouse Ab (A, D, G, and J). The same cells were incubated with rabbit anti-giantin Ab followed by TR-conjugated anti-rabbit Ab (B, E, H, and K). Merged images are shown on the right (C, F, I, and L). Images A–C and G–I represent untreated cells, and images D–F and J–L represent cells treated for 1 h with 20 μM NZ before viewing. Bars, 10 μm.