(A–C) WT and Abcg1–/– mice were put on a chow diet, WTD, or HCD (n = 4–5 per group). (D–F) WT, Abca1–/–, Abcg1–/–, and Abca1–/–Abcg1–/– mice were put on the WTD (n = 5 per group). (A and D) Cholesterol (Chol), (B and E) 7-KC, and (C and F) the ratio of 7-KC to cholesterol were measured. The results are represented as mean ± SEM. *P < 0.05 versus chow diet; †P < 0.05 versus WTD.