Figure 9. Effects of antioxidants and NOS inhibitor on eNOS dimer disruption by 7-KC.

(A–D) HAECs were incubated with 7-KC (10 μg/ml) in the presence of GSH (10 mM), NAC (10 mM), or HDL (100 μg/ml) for 16 h. (A) Western blot for eNOS dimer and monomer. (B) Quantification of the eNOS dimer/monomer ratio. (C) Fluorescence of CM-H₂DCFDA. Original magnification, ×200. (D) Quantification of CM-H₂DCFDA fluorescence. (E and F) HAECs were incubated with 7-KC in the presence or absence of l-NAME for 16 h. (E) Western blot for eNOS dimer and monomer. (F) Quantification of the eNOS dimer/monomer ratio. (G) HAECs were incubated with 7-KC (5–20 μg/ml) in the presence of l-NAME or HDL (100 μg/ml) for 16 h. Western blot for nitrotyrosine. All lanes were run on the same gel but were noncontiguous between 7-KC and 7-KC + l-NAME. The results are represented as mean ± SEM of 3 individual experiments. *P < 0.05 versus vehicle control; ^#P < 0.05 versus 7-KC alone. Veh, vehicle.