Table 4.
Co-expression of validated targets in pancreatic cancer cell lines.
| Target | IL1RAP | PCDHB10 | PTPRR | SLC2A13 | ||||
|---|---|---|---|---|---|---|---|---|
| Cell Line |
mRNA (sem)# |
Protein† | mRNA (sem)# |
Protein† | mRNA (sem)# |
Protein† | mRNA (sem)# |
Protein† |
| AsPC-1 | 1.9 (0.1) | +++ | 13 (3) | +++ | 57 (10) | +++ | 90 (10) | +++ |
| Capan-1 | 12 (2) | +++ | 5.5 (0.8) | +++ | 46 (8) | +++ | 1.5 (0.8) | +++ |
| HPAFII | 0.63 (0.1) | +++ | 2.0 (0.3) | ++ | 26 (5) | +++ | 5.9 (4) | +++ |
| PSN-1 | 0.70 (0.1) | +++ | 0.044 (0.004) | ++ | 0.1 (0.09) | ++ | 11 (0.6) | ++ |
| SU86.86 | 0.62 (0.1) | + | 0.009 (0.005) | ++ | 2.3 (0.1) | ++ | 0.39 (0.1) | ++ |
#
Normalized to β-actin (ACTB) expression [(target gene 2−CT/ACTB 2−CT) * 1000]. Data are the mean of 3 samples and error values are the standard error of the mean (sem).
†
Relative staining intensity as compared to no 1° Ab control: +++ = high, ++ = moderate, + = low. Controls had no staining.