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. Author manuscript; available in PMC: 2009 Jul 30.
Published in final edited form as: J Neurosci Methods. 2008 May 15;172(2):250–254. doi: 10.1016/j.jneumeth.2008.05.00

Figure 1. Quantification of relative abundance using Western Blotting.

Figure 1

Serial dilutions (21.9, 24.3, 27, 30, 33, 37 and 41μg of protein) derived from mouse cortex were run on SDS polyacrylamide gels. Large dotted rectangles represent quantified area (both a long strip or a smaller rectangle worked sufficiently to quantify total protein). Smaller, solid adjacent rectangles represent subtracted background, either between the lanes (for total stains) or below the bands (for single proteins). A: Amido black total protein stain, B: SYPRO® Ruby total protein stain, C: Representative blots stained with anti-PSD-95 D: anti-GAPDH (Santa Cruz.) and E: anti-GAPDH (sigma). F: Optical density at each protein concentration averaged over 10 blots, showing the different “relative abundance” slopes determined using each potential loading control. (β-actin and pERK were omitted for clarity, and because they were represented on <10 blots.) These data were examined statistically in figure 2B.