Figure 3.

CD4⁺CD25⁺ Tregs from the eye of recovering m-EAU rats inhibited CD4⁺CD25⁻ effector T cells. (A) CD4⁺CD25⁻ responder T cells (4 × 10⁵ cells/well) from the spleens of m-EAU rats (9 days p.i.) were mixed at the indicated ratios with CD4⁺CD25⁺ T cells from the eyes or spleens of recovering m-EAU rats (16 days p.i.) and stimulated with R16 (10 μg/mL) presented by irradiated syngeneic spleen APCs (1 × 10⁵/well). T-cell proliferation was measured at 48 hours by [³H]-thymidine uptake and expressed as cpm of triplicate wells. (B) As in (A), but using CD4⁺CD25⁺ T cells from the eyes or spleens of recovering r-EAU rats (12 days p.i.). (C) Percentage inhibition of the proliferation of R16-specific CD4⁺CD25⁻ effector T cells by CD4⁺CD25⁺ Tregs derived from the eyes of recovering m-EAU or r-EAU rats or the spleens of recovering m-EAU rats calculated from (A) and (B). Results shown are representative of those from three independent experiments.