Fig. 5.

TNF-α + PGE2 induce a complex that binds to an IRF1 promoter GAS sequence. (A) Cell extracts were subjected to EMSA using the radiolabeled IRF1 oligonucleotide. (B) The sequence of IRF1 oligonucleotide used in EMSA assays. The core GAS sequence and the extended palindromic sequence are identified. (C) TNF-α + PGE2 mature DC extracts were subjected to EMSA using the radiolabeled IRF1 oligonucleotide and unlabeled wild-type (WT) or mutant oligonucleotides as competitors. (D) Cell extracts were subjected to EMSA using the radiolabeled IRF1 oligonucleotide. Stat1 or Stat5 antibodies were added to extracts for 1 h prior to addition of the radiolabeled probe to supershift Stat-containing complexes.