TABLE 1.
Differential Cytokine Gene Expression in Macrophages and DCs
| Gene access # | Common name | DC1 | DC2 | DC3 | Mac1 | Mac2 | Mac3 |
|---|---|---|---|---|---|---|---|
| NM_010554 | IL-1α | 37.0 | 25.6 | 42.3 | 54.4 | 122.7 | 139.8 |
| NM_008361 | IL-1β | 40.8 | 30.3 | 11.9 | – | 53.0 | – |
| J03783 | IL-6 | 81.0 | 38.0 | 27.3 | 9.4 | 5.8 | 26.4 |
| NM_010548 | IL-10 | 8.5 | 58.3 | 101.2 | 22.8 | 22.9 | 16.1 |
| M86671 | IL-12b | 7.0 | 10.2 | 11.6 | 2.8 | 2.7 | – |
| NM_008357 | IL-15 | 6.2 | 5.8 | 7.0 | 3.6 | 3.3 | 5.3 |
| NM_008360 | IL-18 | 1.9 | 5.7 | – | 3.9 | 19.6 | 9.2 |
| AF301619 | IL-23, α subunit p19 | 15.4 | 4.3 | 10.3 | 10.7 | 1.8 | 1.6 |
| NM_013693 | TNF | 41.0 | 24.6 | 19.7 | 64.6 | 74.1 | 46.5 |
| NM_010798 | macrophage migration inhibitory factor (MIF) | 2.0 | 1.0 | 2.3 | 4.8 | 3.8 | 5.3 |
| NM_010510 | IFN-β | 24.0 | 4.9 | 4.9 | 9.8 | 2.2 | 4.2 |
| NM_010927 | NOS2 | 21.11 | 22.28 | 34.01 | 14.07 | 14.61 | 13.52 |
Total RNA was isolated from uninfected DCs and macrophages and from both cell types after 4 h of M. tuberculosis infection. The RNA was converted to cDNA and subjected to a microarray analysis as described in Materials and Methods. Changes in gene expression are represented as the ratio of signal intensities of infected cells to uninfected cells. Values represent gene expression changes from three separate experiments. – indicates that gene expression of infected and uninfected samples was below the background signal. Mac, Macrophages.