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. 2008 Aug 1;95(9):4361–4371. doi: 10.1529/biophysj.108.138909

FIGURE 5.

FIGURE 5

Isothermal titration calorimetry. ITC binding isotherms for the association of the top strand of the 15 bp TGTGT(15) (A) and CGCGC(15) (B) duplexes containing a single, site-specific 1,3-GNG intrastrand cross-link of transplatin with the complementary (nonmodified) strand (bottom strands of the duplexes TGTGT(15) and CGCGC(15), respectively) at 4°C in 10 mM phosphate buffer (pH 7.0) containing 50 mM NaCl. The upper panels show total heat released on injecting 5 μL aliquots of the 50 μM bottom strand into a 1.4 ml reaction cell containing the 5 μM top strand. The lower panels show the resultant binding isotherm (solid squares) obtained by integrating the peak areas of each injection. The continuous line represents the nonlinear least squares fit of the affinity (K), enthalpy (ΔH), and stoichiometry (n) to a single-site binding model. For other details, see the text.