TABLE 1.
Rearrangement of single, site-specific 1,3-GNG intrastrand cross-links of transplatin in 20-bp duplexes into interstrand cross-links
| Intrastrand cross-linked sequence* | Interstrand CLs after 24 h at 37°C (%) | t50% (h)† | Reference |
|---|---|---|---|
| TGAGT | 91 | 6 | (42) |
| TGTGT | 70 | 12 | (42) and this work |
| 5′-CGTGT | >50 | 6 | (42) |
| 5′-TGTGC | >50 | 18 | (42) |
| 5′-GGTGT | >50 | 4 | (17) |
| AGAGA | >50 | 1.6 | (17) |
| TGAGT | >50 | 4.8‡ | (17) |
| TGGGT | 43 | >24 | this work |
| TGCGT | 29 | >24 | this work |
| CGCGC | 26 | >24 | this work |
| AGCGA | 18 | >24 | this work |
| 5′-CGCGT | 14 | >24 | this work |
| TGCGC | 7 | >24 | this work |
The platinated duplexes (2 μM) were incubated in a medium of 0.2 M NaClO4, 5 mM Tris-HCl, pH 7.5 at 37°C.
*
The central nucleotide sequence of the top strand of the 20-bp duplex at which the 1,3-GNG intrastrand cross-link of transplatin was formed. Gs in bold represent the platinated sites in the intrastrand cross-link.
†
The time at which the rearrangement reached 50%.
‡
This value was obtained for the rearrangement reaction at 40°C.