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. 2008 Jul 25;95(9):4266–4276. doi: 10.1529/biophysj.108.138271

FIGURE 8.

FIGURE 8

Probability density histograms of R13Q-blocked events recorded in the absence and presence of amines. The sampled events were binned and plotted using the square root versus log time format of Sigworth and Sine (see Materials and Methods). Arrows indicate the time constants. (A) Unblocked dwell times recorded from single Na channels in the presence of 8 μM external R13Q. Unblocked events from six different single Na channels were pooled to obtain a sample of 225 events. The solid line is a fit of the data to a single exponential with a time constant of 13.0 s. (B) Unblocked dwell times recorded from single Na channels in the presence of 8 μM external R13Q and 10 mM internal TPrA. Unblocked events from four different single Na channels were pooled to obtain a sample of 214 events. The solid line is a fit of the data to a single exponential with a time constant of 16.3 s. (C) Unblocked dwell times recorded from single Na channels in the presence of 8 μM external R13Q and 10 mM internal DEA. Unblocked events from five different single Na channels were pooled to obtain a sample of 123 events. The solid line is a fit of the data to a single exponential with a time constant of 21.0 s. (D) Blocked dwell times recorded from single Na channels in the presence of 8 μM external R13Q. Blocked events from six different single Na channels were pooled to obtain a sample of 224 events. The solid line is a fit of the data using a single exponential with a time constant of 8.7 s. (E) Blocked dwell times recorded from single Na channels in the presence of 8 μM external R13Q and 10 mM internal TPrA. Blocked events from four different single Na channels were pooled to obtain a sample of 216 events. The solid line is a fit of the data using a single exponential with a time constant of 8.3 s. (F) Blocked dwell times recorded from single Na channels in the presence of 8 μM external R13Q and 10 mM internal DEA. Blocked events from five different single Na channels were pooled to obtain a sample of 123 events. The solid line is a fit of the data using a single exponential with a time constant of 8.0 s.