FIGURE 9.

Inhibition of R13X block by the amines, DEA and TPrA—summary of kinetic changes. (A) τ_unblocked, τ_R13E, and (app) (calculated from Eq. 6) for R13E. (B) τ_unblocked, τ_R13Q, and (app) (calculated from Eq. 6) for R13Q. Fill patterns (insets) denote data taken in the absence of amine; in the presence of 10 mM TPrA; or in the presence of 10 mM DEA. Pairwise testing (t-test, p < 0.05), and ANOVA, both indicated that there was a significant dependence on amine of K_d and τ_unblocked, but not τ_R13Q, for the R13Q experiments. The relatively small apparent differences among the three groups for the R13E experiments did not reach statistical significance by the ANOVA test, consistent with the smaller net charge of R13E.