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Natrin toxin blocks RyR1 calcium efflux from skeletal SR vesicles. (A) Extravesicular [Ca2+] was monitored by the Ca2+ indicator, antipyrylazo III. (Blue) 200 nM thapsigargin in H2O; (green) 200 nM thapsigargin in buffer (30 mM KCl, 25 mM Na-HEPES, pH 7.0); (red) 200 nM thapsigargin + 0.5 μM natrin in buffer (30 mM KCl, 25 mM Na-HEPES, pH 7.0). (B) Averaged maximal rates of calcium efflux with or without natrin. Data are presented as mean ± SE.
